Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition being a selectable marker?

How can we make a host cell competent to receive a foreign gene or DNA?

Selectable markers help us In identifying and eliminating non transformed cells during gene cloning. Explain how the gene for b- galactosidase enzyme works as selectable marker?

Jaya read in a biotechnology book that alien DNA can be introduced into host cell by micro injection and biolistics. Explain these methods

Restriction endonucleases are the enzymes used to cut the DNA molecules. Give any two procedures to introduce the recombinant DNA into the host cell.

Following are the various steps of recombinant DNA technology. Arrange them in correct sequential order. i. Obtaining the foreign gene product ii. Amplification of gene of interest using PCR. iii. Cutting of DNA at specific locations. iv. Downstream processing. v. Insertion of recombinant DNA into the host cell/organism. vi. Isolation of genetic material.