Multiple copies of the gene of interest in synthesized in vitro using DNA polymerase enzyme.
Which are the three steps of the above process?

Multiple copies of the gene of interest is synthesized in vitro using DNA polymerase enzyme. Find out the DNA polymerase enzyme used here.

We can produce multiple copies of gene of interest through a process. Identify the process.

The picture given below shows the technique used for generating multiple copies of the gene of interest. (a) What is the technique called? (b) Name the reactions at step I, step II and step III. (c.) Explain the principle underlying this techniques of DNA amplification.

Use of thermostable DNA polymerase from the bacterium Thermus aquatics,made it possible to generate billion copies of DNA in a very short time using a process. (a) Name the Process. (b) Name the three important steps involved in this process.

Following are the various steps of recombinant DNA technology. Arrange them in correct sequential order. i. Obtaining the foreign gene product ii. Amplification of gene of interest using PCR. iii. Cutting of DNA at specific locations. iv. Downstream processing. v. Insertion of recombinant DNA into the host cell/organism. vi. Isolation of genetic material.