Polymerase Chain Reaction is the synthesis of multiple copies of gene of interest in vitro using two sets of primers and DNA polymerase enzyme. Commonly used DNA polymerase for PCR reaction is Taq polymerase. Write the significance of Taq polymease enzyme.

Use of thermostable DNA polymerase from the bacterium Thermus aquatics,made it possible to generate billion copies of DNA in a very short time using a process. (a) Name the Process. (b) Name the three important steps involved in this process.

The picture given below shows the technique used for generating multiple copies of the gene of interest. (a) What is the technique called? (b) Name the reactions at step I, step II and step III. (c.) Explain the principle underlying this techniques of DNA amplification.

Multiple copies of the gene of interest in synthesized in vitro using DNA polymerase enzyme. Which are the three steps of the above process?

Multiple copies of the gene of interest is synthesized in vitro using DNA polymerase enzyme. Find out the DNA polymerase enzyme used here.

Following are the various steps of recombinant DNA technology. Arrange them in correct sequential order. i. Obtaining the foreign gene product ii. Amplification of gene of interest using PCR. iii. Cutting of DNA at specific locations. iv. Downstream processing. v. Insertion of recombinant DNA into the host cell/organism. vi. Isolation of genetic material.

Answer any 3 question from Q.NO 10 TO 13. Each question carries 3 score. The stage in the productionof human insulin bacteria through the process of genetic engeneering is given. Rearrange correctly. (a) Plasmids (circular DNA) is isolated from a bacterium. (b)This bacteriam is allowed to multiply in a culture medium to produce inactive insulin. (c)From human DNA, cut the gene responsible for the production of insulin. (d) Human insulin gene is ligated with the isolated plasmid, which is used as the vector. (e)Active insulin is produced from this.

Observe the following illustration and answer the questions. Identify the process indicated.