The recombinant DNA technological process have made immense impact in the area of health care. How Eli Lilly Produced Insulin?

Recombinant DNA technology is a complex process which involves several steps. Write down the major steps in recombinant DNA technology.

Following are the various steps of recombinant DNA technology. Arrange them in correct sequential order. i. Obtaining the foreign gene product ii. Amplification of gene of interest using PCR. iii. Cutting of DNA at specific locations. iv. Downstream processing. v. Insertion of recombinant DNA into the host cell/organism. vi. Isolation of genetic material.

A group of students came to know about recombinant DNA technology. They want to know how scientists can produce a new desired product using technology. Can you give them an idea about the important steps that are involved in this process?

Recombinant DNA technology can be accomplished only if we have the following key tools i.e., Restriction enzymes, polymerase enzyme. Ligase and vecors. State the functions of (a) Ligase (b) Restriction enzymes.

Insulin getting assembled into a mature form was the major challenge in commercial insulin production by rDNA technology. How did Eli Nilly Company find a solution to this problem?