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What are the diferent steps of somatic h...

What are the diferent steps of somatic hybridisation?

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1. Select a single cell from a plant with desirable characters.
2. Cell wall of cells are digested by treating the cell with enzyme like pectinase and cellulase to isolate protoplast.
3. The isolated protolast of two different varieties of plant cells can be fused to get hybrid protoplast.
4. The hybrid protoplast allows to regenerate cell wall.
5. Then it can be further grown to form new plant called somatic hybrid.
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