Describe briefly the following:
Origin of replication

Explanation

It is the sequences of bases on the DNA from where the replication starts and when a segment of foreign DNA linked to this segments, the forein DNA will replicate along with the rest of the vector DNA.
This sequence is also responsible for controlling the number of copies of the foreign DNA.
To obtain a large number of copies of the foreign DNA the cloning should be done in a vector which has an origing supporting high copy production.