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Each restriction endonuclease recognizes...

Each restriction endonuclease recognizes a specifi palindromic nucleotide sequences in the DNA. Write the palindrome in DNA recognised by EcoRI.

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5. GAATTC 3.
3. CTTAAG 5.
The above base sequences in the DNA fragments reads the same on the two strands in direction and same on the two strands in direction.
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Each restriction endonuclease recognizes a specifi palindromic nucleotide sequences in the DNA. What is meant by palindrome in DNA.

Write the specific palindromic nucleotide sequene in DNA that is recognised by Eco RI

Knowledge Check

  • Assertion : Each restriction endonuclease recognises a specific palindrome nucleotide sequences in the DNA . Reaso : the palindrome in DNA is a sequence of base pairs that reads same on the two strands when orientation of reading kept the same .

    A
    If both assertion and reason are true and the reason is the correct explanation of the assertion .
    B
    If both assertion and reason are true but reason is not the correct explanation of the assertion .
    C
    If assertion is true but reason is false .
    D
    If both assertion and reason are false .

  • Assertion : Each restriction endonuclease functions by inspecting the length of a DNA sequence . Reason : Once it finds its specific recognition sequence , it will bind to the DNA and cut hydrogen bonds .

    A
    If both assertion and reason are true and the reason is the correct explanation of the assertion .
    B
    If both assertion and reason are true but reason is not the correct explanation of the assertion .
    C
    If assertion is true but reason is false .
    D
    If both assertion and reason are false .

  • Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from:

    A
    Plasmids
    B
    All prokaryotic cells
    C
    Bacteriophages
    D
    Bacterial cell

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    Restriction Endonucleases are enzymes responsible for cutting of DNA molecule. Eco RI is a common example. Write the palindromic sequence of Eco RI.

    DNA finger printing is a technique in molecular biology. Arrange the following steps in sequence. Blotting of DNA fragment to nitro cellulose. Digestion of DNA by restriction endonuclease. Deletion of DNA by restriction endonuclease. Isolation of DNA, separation of DNA fragments by electrophoresis.

    Restriction endonucleases are the enzymes used to cut the DNA molecules. Name the enzyme that joins the foreign DNA and vector DNA.

    Restriction endonucleases are the enzymes used to cut the DNA molecules. Give any two procedures to introduce the recombinant DNA into the host cell.

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