Mention the different steps of process of recombinant DNA technology.

Explanation

a. Isolation of DNA
b. Fragmentation of DNA by restriction endonucleases.
c. Isolation of a desired DNA fragment.
d. Ligation of the DNA fragment into a vector.
e. Insertion of recombinant DNA into the hast.
f. Culturing the host cells on a suitable medium at a large scale.
g. Extraction of the desired gene product.
h. Down stream processing of the products as finished product, ready for marketing.