Define origin replication.

Assertion : All expression vectors are cloning vectors and vise versa. Reason : Expression vectors have at least the regulatory sequences i.e., promotes, operators, ribosomal binding sites, etc having optimum function in the chosen control but not origin of replication.

The enzyme called_____bind to the origin of replication site.

Explain the significance of origin of replication and selectable market in gene cloning experiments .

Which one of the following characteristics is generally not preferred for a cloning vector ? a) An origin of replication b) An antibiotic resistance marker c)Multiple restriction sites d) A high copy number

{:(1.,"Homozygous",-,(a),"polynucleotide"),(2.,"Genes",-,(b),"human male"),(3.,"DNA",-,(c),"occurs in pairs and alike"),(4.,"Heterozygous",-,(d),"segments of DNA"),(5.,"Heterogametic",-,(e),"bind DNA to the origin of replication site"),(6.,"Autosome",-,(f),"occurs in pairs and unlike"),(7.,"Helicase",-,(g),"somatic characters"):}

Define replication of DNA.

In which organism, 400 origins of replication can be found _____

Describe briefly the following : a. Origin of replication b. Bioreactors c. Downstream processing