What is Taq polymerase?

During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme 1) α -glucosidase 2) restriction endonuclease 3) β -galactosidase 4) Taq polymerase

Which of the following contains the key tools for recombinant DNA technology? (i) Restriction endonucleases, ligases, vectors (ii) Ligases, host organism, ligases, vectors (iii) Vectors, Taq polymerase, primers (iv) Restriction exonucleases, ligases, primers, bioreactors

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

In a polymerase chain reaction after the denaturation step why the mixture needs to cool down to a lower temperature ? a) To permit specific annealing of the primers b) To give a halt to the reaction mixture c) To increase the activity of enzyme Taq polymerase d) To obtain the multiple copies of the DNA

What is meant by polymerase chain reaction(PCR) ?

What are the two major components of prokaryotic RNA polymerase? How do they act?

Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the high temperature induced denaturation of dsDNA. (ii) It requires primers for carrying out the process of polymerisation. (iii) It synthesises the RNA region between the primers, using dNTPs and Mg^(2+) .

These questions consists of two statements each printed as Assertion and Reason. Choose any one of the following response to answer these questions. Both Assertion and Reasort are true and Reason is correct explanation of Assertion.Both Assertion and Reason are true but Reason is not correct explanation of Assertion . Assertion is true but Reason is false Both Assertion and Reason are false: Assertion : During primer extension, the PCR mixture is heated to 75^@C to extend each primer by copying the single stranded template. Reason : The primary template synthesis DNA by using Taq-polymerase.

Process used for amplification or multiplication of DNA in DNA fingerprinting is 1) polymerase chain reaction 2) southern blotting 3) northern blotting 4) None of these

The taq polymerase enzyme is obtained from: