What is meant by recombinant DNA technology ?

How and why is the bacterium Thermus aquaticus employed in recombinant DNA technology ? Explain .

Second generation vaccines are prepared by recombinant DNA technology. Which out of the following are the examples of such vaccines ?

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Separation and isolation of DNA fragments is a major event in recombi-nant DNA technology . The separated fragments cannot be seen in a nor-mal way . Is there any method to visualise the separated DNA ? Explain .

Read the given statements and select the correct option. Statement 1: Both bacteria and yeast multiply very fast to form huge populations which express the desired gene. Statement 2: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryotes).

Plasmid acts as a _______in recombinant DNA technology.

Mention the role of vectors in recombinant DNA technology . Give any two examples .