Define replication fork.

Define replication of DNA.

…………………is the enzyme, which separates the double helix of DNA, above the replication fork.

A_(3)-_(5)B C_(5)-_(3)D AB and CD represent two strands of a DNA molecule. When this molecule undergoes replication, a replication fork is formed between A and C in the above. i. Name the template strands for replication. ii. Using which strand as the template, will there be continuous synthesis of a complementary DNA strand? iii. Complementary to which strand will Okazaki segments get synthesised. iv. What are template strands and Okazaki pieces? v. In which direction is a new strand synthesised?

An enzyme which removes supercoiling from replication fork during replication _____

What is a replication fork?

Redraw the structure as a replicating fork and label the parts.

Define origin replication.

i. Southern blotting - Probe ii. beta-gal - Lac operon iii. Replication fork - Ligase iv. Singer - TMR

a) Identify the figure given below b) Redraw the structure as a replicating fork and label the parts c) Write the source of energy for this replication and name the enzyme involved in this process. d) Mention the differences in the synthesis of protein, based on the polarity of the two template strands.