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What are the enzymes you can used to cut...

What are the enzymes you can used to cut terminal end and internal phospho di ester bond of nucleotide sequence?

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Assertion (A): Arabidopsis plant chromosome have more repeats of TTTAGGG nucleotide sequence in the telomers. Reason (R ): Restriction endonuclease enzyme is used in the formation of such a nucleotide sequence.

Which of the following statements does not hold true for restriction enzyme? (a) It recongnises a palindromic nucleotide sequence (b) It is an endonuclease. (c) It is isolated from viruses (d) It produces the same kind of sticky ends in different DNA molecules

You are working in a biotechnology lab with a bacterium namely E.coil. How will you cut the nucleotide sequence? Explain it.

Rinku with a circular DNA contains sequence 5' larr GGATTCC to 3' 3' larr CCTTAAGG to 5' She wishes to add a new segment of DNA into it. a. Identify the technology she planned. b. Suggest the specific enzyme to make a cut in the DNA with above sequence. c. Name the ctegory of enzyme your suggested. d. How this enzyme identifies the sequence? e. Draw the cut ends of the DNA with sequence.

Read the following paragraph carefully and answer the following: Tie a stone to one end of a string. Take the other end in your hand and rotate the string so that the stone moves along a circle. As long as we are holding the string, we are pulling the stone towards us i.e. towards the centre of the circle and are appliying a force towards it. The force stops acting on it if we release the string. In this case, the stone will fly off along a straight line which is the tangent to the circle at the position of the stone when the string is released, because that the directionn of its velocity at that instant of time. You may recall a similar activity in which a 5 rupee coin kept on a rotating circular disc flies off the disc along the tangent to the disc. Thus, a force acts on any object moving along a circle and it is directed towards the centre of the circle. This is called the Centripetal force. What happens if the string is released?

SARAS PUBLICATION-PRINCIPLE AND PROSSES OF BIOTECHNOLOGY-EXERCISE
  1. EcoRI cleaves DNA at

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  2. Consider the following statements : i. Recombinant DNA technology is...

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  3. In which techniques Ethidium Bromide is used?

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  4. 5-Bromo-4indolu-beta-D-galactopyranoside is called

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  5. Bt brinjal is created by insertine a crystal protein gene called

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  6. What are the enzymes you can used to cut terminal end and internal pho...

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  7. Name the chemicals used in gene transfer.

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  8. Write the risk of genetically modified food.

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  9. Is their any possibilities to tranfer a suitable desirable gene to hos...

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  10. What are the main features that are required to facilitate cloning in...

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  11. Define Biopharming . Give its uses .

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  12. You are working in a biotechnology lab with a bacterium namely E.coil....

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  13. What do you know about the word pBR332?

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  14. What is bioremediation? Give some exapmles of bioremediation.

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  15. Name and describe the blotting technique which is used to separate t...

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  16. Name and explain the plasmid which is found in Agrobacterium tumefacie...

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  17. What are restriction enzymes or molecular scissors?

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  18. What are restrictions enzyme. Mention their type with role in biotechn...

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  19. What do you mean by DNA probes ?

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  20. Explain the replica plating technique .

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