Vehicle or Vector DNA || Origin OF Replication || Selectable Marker || Cloning Site || Size OF Vector

Characteristics OF Cloning Vectors || Origin OF Replication || Selectable Markers || Recognition (=cloning) Sites || selection OF non-transformants from transformants and Recombinants From Non-Recombinants || Insertional Inactivation

Characteristics OF Cloning Vectors:- 1.Origin OF Replication 2. Selectable Markers 3.Recognition (=cloning) Sites, selection OF non-transformants from transformants and Recombinants From Non-Recombinants, Insertional Inactivation

Which of the following features are required to facilitate cloning into a vector ? a. Origin of replication (ori) b. Selectable marker c. Cloning site

Tools OF rDNA Technology|| Vectors:- a)Ori b)Selectable Marker c)Cloning Sites || Examples And Types OF Vector || PBR322 Vector

Cloning Vectors

Tools OF rDNA Technology, Vectors:- a)Ori b)Selectable Marker c)Cloning Sites d) Examples And Types OF Vector e)PBR322 Vector

Marker gene in vector

Assertion : Origin of replication (ori) is a definite region in E. coli DNA where the replication originates. Reason : The vectose in r-DNA procedures provide the origin of replication.

Cloning vector contains