Tools OF Genetic Engineering, Competent Host, Types OF Gene Transfer:-a)Vector Mediated Transfer,b) Vector Less Transfer ,Insertional Inactivation, Selecting Transformant and Non-Transformant and Between Recombinant and Non-Recombinant

Tools OF Genetic Engineering || Competent Host || Types OF Gene Transfer:-a)Vector Mediated Transfer || Vector Less Transfer || Insertional || Inactivation || Selecting Transformant and Non-Transformant || Recombinant and Non-Recombinant

Competent Host, Types OF Gene Transfer, GenBank, Gene Library ,Cryopreservation

Competent Host || Types OF Gene Transfer || GeneBank || Gene Library || Cryopreservation

Vectors, Recombinant DNA technology Culturing of Host, Screening of recombinant and non-recombinants , DNA transfer method and Recombinant Protein

Characteristics OF Cloning Vectors:- 1.Origin OF Replication 2. Selectable Markers 3.Recognition (=cloning) Sites, selection OF non-transformants from transformants and Recombinants From Non-Recombinants, Insertional Inactivation

Characteristics OF Cloning Vectors || Origin OF Replication || Selectable Markers || Recognition (=cloning) Sites || selection OF non-transformants from transformants and Recombinants From Non-Recombinants || Insertional Inactivation

In the technique of selection of transformed , recombinant host cell by insertional inactivation method, the correct statements is .

Assertion: Genetic engineering is essentially the alteration of the genetic make up of cells by deliberate and artificial means. Reason: It involves transfer or replacement of genes to create recombinant DNA.

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as