Processes in rDNA Technology:- Amplification OF Desired DNA fragment (PCR plymerase Chain Reaction), Ligation OF Desired DNA With Vector, transformation OF Host cell With rDNA

Processes in rDNA Technology:- Amplification OF Desired DNA fragment (PCR plymerase Chain Reaction) || Ligation OF Desired DNA With Vector || Transformation OF Host cell With rDNA

Processes OF rDNA Technology:- Introduction, Isolation OF DNA, Fragmentation OF DNA by RE, Separation and Isolation OF Desired DNA Fragment (Agarose Gel Electrophoresis)

Processes OF rDNA Technology:- Introduction || Isolation OF DNA || Fragmentation OF DNA by RE || Separation and Isolation OF Desired DNA Fragment (Agarose Gel Electrophoresis)

Amplification OF the Gene OF Interest using PCR|| Ligation OF the DNA fragment into a Vector|| Insertion OF Recombinant DNA into the Host cell/organism

One of the methods by which DNA connot be transformed to the host cell is by :

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Processes in rDNA Technology:- a)Culture OF Transformed Host Cells on Large Scale-Bioreactors,Obtaining Product-Downstream Processing

The Process OF Recombinant DNA Technology || Isolation OF Genetic Material || Fragmentation OF DNA at a specific location by RE || Separation OF Isolation OF DNA Fragments

Which of the following statement // s regarding nucleic acids is // are wrong ? Amplification of gene of interest is done by polymerase Chain Reaction. Origin of replication (ori) is a sequence from where RNA replication starts. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens can be modified into a cloning vector . DNA technology involves the use of restriction endonucleases. Biolistics or gene gun is suitable to introduce DNA to plant cells.

The cells of E. coli may be made competent to take up external DNA in the process of transformation by expos- ing the cell to: