Processes in rDNA Technology:- a)Culture OF Transformed Host Cells on Large Scale-Bioreactors,Obtaining Product-Downstream Processing

Processes in rDNA Technology || Culture OF Transformed Host Cells on Large Scale || Bioreactors|| Obtaining Product-Downstream Processing

Obtaining the Foreign Gene Product:- Bioreactors, Downstream Processing

Obtaining the Foreign Gene Product || Bioreactors and Downstream Processing

Describe briefly the following (a) Bioreactors (b) Downstream processing

Polymerase chain reaction || culture OF host || bioreactors || downstream process

Processes in rDNA Technology:- Amplification OF Desired DNA fragment (PCR plymerase Chain Reaction) || Ligation OF Desired DNA With Vector || Transformation OF Host cell With rDNA

Processes in rDNA Technology:- Amplification OF Desired DNA fragment (PCR plymerase Chain Reaction), Ligation OF Desired DNA With Vector, transformation OF Host cell With rDNA

Describe briefly the following: (a) Origin of replication. (b). Bioreactors. (c). Downstream processing.

From given statements select the incorrect statement. (a) Proteins which are obtained by recombinant DNA technology are called recombinant proteins (b) Downstream processing include separation and purification of the desired product (c) After purification the product has to be formulated with suitable preservatives (d) Downstream processing and quality control testing do not vary from product to product

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.