Which of the followng is used in tissue culture :-

Options

A. Cow milk
B. Buffalow milk
C. Goat milk
D. Coconut milk

Answer

### Step-by-Step Solution:

1. **Understanding Tissue Culture**:
- Tissue culture is a technique used to grow plant cells, tissues, or organs in a controlled environment, usually on a nutrient medium. The process relies on the concept of totipotency, where a single plant cell can regenerate into a whole plant.

2. **Identifying the Options**:
- The options given are cow milk, buffalo milk, goat milk, and coconut milk. We need to determine which of these is used in the tissue culture process.

3. **Analyzing the Options**:
- Cow milk, buffalo milk, and goat milk are animal products and are not typically used in plant tissue culture.
- Coconut milk, on the other hand, is derived from the endosperm of the coconut fruit and is known to be rich in nutrients.

4. **Understanding the Role of Coconut Milk**:
- Coconut milk contains cytokinins, particularly kinetin, which are plant hormones that promote cell division and growth. This makes coconut milk a suitable medium for supporting the growth of plant tissues in culture.

5. **Conclusion**:
- Based on the analysis, coconut milk is the correct answer as it is used in tissue culture due to its beneficial properties for plant growth.

### Final Answer:
Coconut milk is used in tissue culture.

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