How many example given below are the example of in-situ conservation? Tissue culture, pollen bank, national park, Biosphere reserve, Botanical garden

Options

A. Three
B. Two
C. One
D. Five

Answer

To determine how many examples from the list are instances of in-situ conservation, we need to analyze each option provided:

1. **Tissue Culture**: This is a laboratory technique used to grow cells or tissues in a controlled environment. It does not occur in the natural habitat of the organisms, so it is **not** an example of in-situ conservation.

2. **Pollen Bank**: A pollen bank is a facility where pollen is stored for future use, often for breeding or conservation purposes. Since it is a man-made facility and does not conserve organisms in their natural habitat, it is also **not** an example of in-situ conservation.

3. **National Park**: A national park is a protected area that conserves the natural environment and wildlife in their natural habitats. Therefore, this is an example of in-situ conservation.

4. **Biosphere Reserve**: Similar to national parks, biosphere reserves are areas designated to promote sustainable development and conservation of biodiversity in natural habitats. Thus, this is also an example of in-situ conservation.

5. **Botanical Garden**: A botanical garden is a man-made area where plants are cultivated for research, education, and display. Since it is not a natural habitat and involves human management, it is **not** an example of in-situ conservation.

Now, summarizing the analysis:
- In-situ conservation examples from the list: **National Park** and **Biosphere Reserve**.
- Total number of in-situ conservation examples: **2**.

### Final Answer:
There are **2** examples of in-situ conservation from the list provided: National Park and Biosphere Reserve.