You have chosen a plasmid as vector for cloning your gene. However, this vector plasmid lacks a selectable marker. How would it affect your experiment?

Why are cloning vectors bacteriophages sometimes preferred over bacterial plasmids ?

The linking of antibiotic resistance gene with the plasmid vector became possible with :

What is called the technique of insertion of a desired gene into DNA of plasmid vector?

The given figure is the diagrammatic representaton of the E. coli vector pBR322. Which of these genes can act as selectable marker ?

Assertion : All expression vectors are cloning vectors and vise versa. Reason : Expression vectors have at least the regulatory sequences i.e., promotes, operators, ribosomal binding sites, etc having optimum function in the chosen control but not origin of replication.

What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector ?

Read the given statements and select the correct option. Statement 1 : The tumour inducing plasmid (Tiplasmid) acts as a cloning vector in recombinant DNA technology. Statement 2: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.