What is called the technique of insertion of a desired gene into DNA of plasmid vector?

Who discovered the plasmid vectors ?

Describe the technique of separation of DNA fragments by electrophoresis.

Name the technique used for the separation of DNA fragments.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Some of the steps involved in the production of humulin are given below. Choose the correct sequence. (i) Synthesis of gene (DNA for human insulin artificially. (ii) Culturing recombinant E.coli in bioreactors. (iii) Purification of humulin. iv Insertion of human insulin gene into plasmid (v) Introduction of recombinat plasmid into E.coli (vi) Extraction of recombinant gene product from E.coli.

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.

What type of technique is used for amplification of DNA ?