In order to obtains virus plants through tissue culture the best method is

Options

A. meristem culture
B. protoplast culture
C. embryo rescue
D. anther culture

Explanation

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for the best method to obtain virus-free plants through tissue culture.

2. **Analyzing the Options**: The options provided are:
- Meristem culture
- Protoplast culture
- Embryo rescue
- Anther culture

3. **Explaining Meristem Culture**:
- Meristematic tissues are regions in plants where cells are actively dividing.
- These tissues lack a vascular system, which is crucial for the movement of viruses within the plant.
- Because of the rapid cell division in meristematic tissues, viruses cannot spread effectively, making it an ideal source for obtaining virus-free plants.

4. **Metabolic Activity**:
- The high metabolic rate in meristematic tissues further inhibits the replication of viruses.
- The presence of plant hormones like auxins in these tissues also helps in preventing virus multiplication.

5. **Evaluating Other Options**:
- **Protoplast Culture**: This method is used for obtaining genetically superior species but does not specifically target virus elimination.
- **Embryo Rescue**: This technique is used for rescuing embryos that would not survive under normal conditions, not for virus elimination.
- **Anther Culture**: This is primarily used for ensuring better pollination and does not relate to virus-free plant production.

6. **Conclusion**: Based on the analysis, the best method to obtain virus-free plants through tissue culture is **meristem culture**.

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