Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used

Options

A. on plates with and without streptomycin
B. on plates with minimal medium
C. only on plates with streptomycin
D. only on plates without streptomycin

Explanation

To solve the question regarding the selection of streptomycin-resistant mutants using imprints from a plate with complete medium carrying bacterial colonies, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Context**: The question involves selecting streptomycin-resistant mutants from a bacterial population. These mutants arise due to random mutations and not as an adaptive response to streptomycin.

2. **Identifying the Purpose of Imprints**: The imprints taken from the original plate with bacterial colonies will be used to transfer the bacteria onto a new medium. The goal is to identify which bacteria are resistant to streptomycin.

3. **Choosing the Right Medium**: To select for streptomycin-resistant mutants, the imprints must be transferred to a medium that contains streptomycin. This is crucial because only the resistant mutants will survive and grow on this medium.

4. **Analyzing the Options**:
- **Option 1**: A plate with complete medium (without streptomycin) - This will not help in selecting resistant mutants since both resistant and non-resistant bacteria will grow.
- **Option 2**: A plate with a different antibiotic - This is not relevant to selecting for streptomycin resistance.
- **Option 3**: A plate with streptomycin - This is the correct option, as only the streptomycin-resistant mutants will grow.
- **Option 4**: A plate without streptomycin - Similar to option 1, this will not help in distinguishing between resistant and non-resistant bacteria.

5. **Conclusion**: The imprints need to be used on a plate containing streptomycin (Option 3) to effectively select for streptomycin-resistant mutants.

### Final Answer:
The imprints need to be used on a plate with streptomycin (Option 3).

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