During translation initiation in prokaryotes, a GTP molecule is needed in `:`

Options

A. association of 30S , mRNA with formly met tRNA0
B. association of 50S subunit of ribosome with initiation complex
C. formation of formaly met tRNA
D. binding of 30S subunit of ribosome with mRNA

Explanation

**Step-by-Step Solution:**

1. **Understanding Translation Initiation in Prokaryotes:**
- Translation is the process where ribosomes synthesize proteins based on the sequence of mRNA. In prokaryotes, this process begins with the initiation phase.

2. **Role of GTP in Translation Initiation:**
- GTP (Guanosine Triphosphate) is a molecule that provides energy for various cellular processes, including translation. During the initiation phase, GTP is specifically used to facilitate certain steps.

3. **Identifying the Correct Step:**
- The question asks for the specific step during translation initiation in prokaryotes where a GTP molecule is needed. The options provided include:
- Association of 30S mRNA with formyl methyl tRNA
- Association of 50S subunit of the ribosome with the initiation complex
- Formation of formyl methyl tRNA
- Binding of 30S subunit with the ribosome mRNA

4. **Analyzing Each Option:**
- **Association of 30S mRNA with formyl methyl tRNA:** This is the step where the 30S ribosomal subunit binds to the mRNA and the initiator tRNA (formyl methionine tRNA). GTP is required for this association.
- **Association of 50S subunit of the ribosome with the initiation complex:** This step occurs after the initial binding and does not directly require GTP.
- **Formation of formyl methyl tRNA:** This step involves the charging of tRNA and does not require GTP.
- **Binding of 30S subunit with the ribosome mRNA:** While this is part of the initiation process, the specific requirement for GTP is during the association with the formyl methyl tRNA.

5. **Conclusion:**
- The correct answer is that a GTP molecule is needed for the **association of 30S mRNA with formyl methyl tRNA** during translation initiation in prokaryotes.

**Final Answer:**
- A GTP molecule is needed for the **association of 30S mRNA with formyl methyl tRNA** during translation initiation in prokaryotes.

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