Organelles can be separated from cell homogenate through

Options

A. chromatography
B. X-rays diffraction
C. differential centrifugation
D. auto -radiography

Explanation

To answer the question "Organelles can be separated from cell homogenate through," we need to analyze the options provided and identify the correct method for separating organelles.

### Step-by-Step Solution:

1. **Understanding Cell Homogenate**:
- A cell homogenate is a mixture created by breaking down cells to release their contents, including organelles.

2. **Identifying Separation Techniques**:
- We need to evaluate the methods listed in the options to determine which one is suitable for separating organelles based on their physical properties.

3. **Evaluating the Options**:
- **A. Chromatography**: This technique separates substances based on their movement through a medium. It is not suitable for separating organelles since it relies on the migration rates of molecules rather than their physical characteristics.
- **B. X-ray Diffraction**: This method is used to study the structure of crystalline materials and is not applicable for separating biological organelles.
- **C. Differential Centrifugation**: This technique involves spinning the homogenate at various speeds. Different organelles will settle at different rates based on their size, mass, and specific gravity, allowing for effective separation.
- **D. Autoradiography**: This technique is used to visualize the distribution of radioactive materials in a sample and is not a method for separating organelles.

4. **Conclusion**:
- Based on the analysis, the correct answer is **C. Differential Centrifugation**. This method effectively separates organelles based on their physical properties.