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The correct order of steps in polymerase...

The correct order of steps in polymerase chain reaction (PCR) is

A

Extension, Denaturation, Annealing

B

Annealing, Extension, Denaturation

C

Denaturation, Extension, Anneasling

D

Denaturation, Annealing, Extension

Text Solution

Verified by Experts

The correct Answer is:
D

Denaturation of double stranded DNA is followed by annealing of short oligonucleotide chains called primers and then as last the DNA polymerase extends or elongates the newly formed strand and thus amplifies the amount of DNA.
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