During translation initiation in prokaryotes, a GTP molecule is needed in `:`

Options

A. Formation of formyl-met-tRNA
B. Binding of 30S subunit of ribosome with mRNA
C. Association of 30 S-mRNA with formylmet- tRNA
D. Association of 50 S subunit of ribosome with initiation complex

Answer

To solve the question regarding the role of GTP during translation initiation in prokaryotes, we can break down the process step by step:

### Step-by-Step Solution:

1. **Understanding Translation Initiation**:
- Translation initiation in prokaryotes involves the assembly of the ribosome, mRNA, and the initiator tRNA. This is a critical step for protein synthesis.

2. **Role of GTP**:
- GTP (Guanosine Triphosphate) is a molecule that provides energy and is involved in various stages of translation. It is particularly important during the initiation phase.

3. **Formation of the Initiation Complex**:
- The initiation complex in prokaryotes consists of the small ribosomal subunit (30S), the mRNA, and the initiator tRNA (formylmethionine tRNA or fMet-tRNA).

4. **Binding of the 30S Subunit**:
- The 30S ribosomal subunit binds to the mRNA. This binding is facilitated by GTP, which helps stabilize the interaction.

5. **Association of fMet-tRNA**:
- Once the 30S subunit is bound to the mRNA, the formylmethionine tRNA (fMet-tRNA) associates with the 30S ribosomal subunit. This step is also dependent on GTP.

6. **Completion of the Initiation Complex**:
- After the association of fMet-tRNA, the 50S ribosomal subunit then joins the complex, completing the formation of the functional ribosome ready for elongation.

7. **Conclusion**:
- Therefore, the specific role of GTP during translation initiation in prokaryotes is most crucial during the association of the 30S ribosomal subunit with mRNA and fMet-tRNA.

### Final Answer:
The correct answer is **C: association of 30S mRNA with formyl Met tRNA**.

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