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The correct order of steps in Polymerase...

The correct order of steps in Polymerase Chain reaction (PCR) is

A

Denaturation, Extension, Annealing

B

Annealing, Extension, Denaturation

C

Extension, Denaturation, Annealing

D

Denaturation, Annealing, Extension

Text Solution

Verified by Experts

The correct Answer is:
D
This technique is used for making multiple copies of gene (or DNA) of interest in vitro. Each cycle has three steps
(i) Denaturation
(ii) Primer annealing
(iii) Extension of primer
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