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In Recombinant DNA technology antibiotic...

In Recombinant DNA technology antibiotics are used:

A

to keep medium bacteria free

B

to detect alien DNA

C

to impart disease-resistance to the host plant

D

as selectable markers

Text Solution

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The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding Recombinant DNA Technology**: This technology involves combining DNA from different organisms. It is used to create genetically modified organisms (GMOs) and to produce proteins, hormones, and other substances. 2. **Role of Antibiotics**: In recombinant DNA technology, antibiotics play a crucial role in the selection of host cells that have successfully taken up the recombinant DNA. 3. **Selectable Markers**: Antibiotic resistance genes are used as selectable markers. These genes allow for the identification of cells that have incorporated the foreign DNA. For example, if a plasmid containing an antibiotic resistance gene is introduced into a bacterial culture, only those bacteria that have taken up the plasmid will survive in the presence of the antibiotic. 4. **Examples of Antibiotics Used**: Common antibiotics used in this context include ampicillin and tetracycline. Plasmids like PBR322 contain genes that confer resistance to these antibiotics. 5. **Conclusion**: The primary use of antibiotics in recombinant DNA technology is as selectable markers to identify and select for bacteria that have successfully incorporated the recombinant DNA. **Final Answer**: The correct answer is option D: as selectable markers. ---
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