In a mixture,DNA fragments are separated by:

Options

A. Bioprocess engineering
B. Restriction digestion
C. Electrophoresis
D. Polymerase Chain Reaction

Answer

To solve the question regarding how DNA fragments are separated in a mixture, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Context**:
- The question asks about the method used to separate DNA fragments in a mixture.

2. **Identifying the Technique**:
- The technique used for separating DNA fragments is called **electrophoresis**. This is a widely used method in molecular biology.

3. **Principle of Electrophoresis**:
- DNA molecules are negatively charged due to the phosphate backbone. When an electric field is applied, these negatively charged DNA fragments will migrate towards the positively charged electrode (anode).

4. **Medium Used**:
- The separation occurs in a gel medium, commonly **agarose gel**. This gel acts as a sieve, allowing smaller DNA fragments to move faster than larger ones.

5. **Movement of DNA Fragments**:
- As the electric current is applied, the DNA fragments will move through the gel. Smaller fragments will travel further and faster through the gel compared to larger fragments due to the sieving effect of the gel.

6. **Conclusion**:
- Therefore, the correct answer to the question is that DNA fragments are separated by **electrophoresis**.

### Final Answer:
The DNA fragments in a mixture are separated by **electrophoresis**.

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