Spooling is:-

Options

A. Amplification of DNA
B. Cutting of seperated DNA bands from the agarose gel
C. Transfer of separated DNA framents to synthetic membranes
D. Collection of isolated DNA

Answer

**Step-by-Step Solution:**

1. **Understanding the Term "Spooling":**
- Spooling refers to a specific laboratory technique used in molecular biology to collect DNA. It is not related to amplification or cutting of DNA.

2. **Differentiating from Other Processes:**
- **Amplification of DNA:** This process is known as PCR (Polymerase Chain Reaction), which is used to amplify DNA samples.
- **Elution:** This term refers to the process of cutting or extracting separated DNA bands from agarose gel, which is not what spooling is about.
- **Blotting:** This is the process of transferring separated DNA fragments to synthetic membranes, which also does not relate to spooling.

3. **Defining Spooling:**
- Spooling is specifically the collection of isolated DNA. This is achieved by wrapping the DNA around a glass rod.

4. **How Spooling Works:**
- The glass rod is dipped into the solution containing isolated DNA. As the rod is pulled out, the DNA wraps around it, allowing for the collection of the DNA.

5. **Conclusion:**
- Therefore, spooling is defined as the collection of DNA by wrapping it around a glass rod, making option D the correct answer.

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