Represent diagrammatically the E.coli cloning vector pBR322.

The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component (s) ?

The given figure is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain component (s)?

Mention the rule of (i) selectable marker, (ii) Ori and (iii) rop in E. coli cloning vector pBR322.

Identify A,B,C, and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

Cloning vector contains

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites,Ori, antibiotic resistance genes& ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

Write the role of 'Ori' and 'restriction' site in a cloning vector pBR322.

Which of the following is correct about pBR322 ?

(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play. (b) Why is the coding sequence of an enzyme b-galactosidase a preferred selectable marker in comparison to the ones named above ?