The release of oxygen by haemoglobin for use in body tissues is dependent on all of the following except:

Options

A. low `P_(O_2)` of body tissue cells
B. alkaline pH of blood in tissue capillaries
C. presence of ATP
D. all of the above cause the release of oxygen from haemoglobin

Explanation

To solve the question regarding the release of oxygen by hemoglobin for use in body tissues, we need to identify which factor does not influence this process. Here’s a step-by-step breakdown:

### Step 1: Understanding Hemoglobin's Role
Hemoglobin is a protein found in red blood cells (RBCs) that carries oxygen from the lungs to the tissues and returns carbon dioxide from the tissues back to the lungs. It has a high affinity for oxygen when the partial pressure of oxygen (PO2) is high, such as in the lungs.

**Hint:** Recall the function of hemoglobin and its affinity for oxygen in different environments (high vs. low PO2).

### Step 2: Factors Influencing Oxygen Release
Several factors affect the release of oxygen from hemoglobin:
- **Partial Pressure of Oxygen (PO2):** Low PO2 in tissues promotes the release of oxygen.
- **Partial Pressure of Carbon Dioxide (PCO2):** High levels of CO2 in tissues can enhance oxygen release.
- **pH Levels (H+ Concentration):** A lower pH (more acidic) promotes oxygen release, while a higher pH (more alkaline) promotes oxygen binding.
- **Temperature:** Higher temperatures can facilitate the release of oxygen from hemoglobin.

**Hint:** Consider how changes in these factors (PO2, PCO2, pH, and temperature) can affect hemoglobin's ability to release oxygen.

### Step 3: Analyzing the Options
Now, we analyze the options given in the question:
- **Option A:** PO2 in tissues - This is a key factor; low PO2 promotes oxygen release.
- **Option B:** Alkaline pH of blood in tissue capillaries - High pH (alkaline) means low H+ concentration, which promotes oxygen binding rather than release.
- **Option C:** ATP energy - While ATP is critical for cellular processes, it does not directly influence oxygen release from hemoglobin.
- **Option D:** All of the above - This option implies that all previous factors affect oxygen release, which is incorrect.

**Hint:** Identify which of the options directly contradicts the process of oxygen release from hemoglobin.

### Step 4: Conclusion
Based on the analysis, the factor that does not promote the release of oxygen from hemoglobin is **Option B: Alkaline pH of blood in tissue capillaries**. A high pH would favor the binding of oxygen to hemoglobin rather than its release.

**Final Answer:** Option B