**Step-by-Step Solution:**
1. **Definition of A-band and I-band:**
- The A-band (Anisotropic band) is the dark band in a muscle fiber that contains thick myosin filaments and overlapping parts of thin actin filaments.
- The I-band (Isotropic band) is the light band that contains only thin actin filaments.
2. **Appearance:**
- The A-band appears dark under a microscope due to the presence of myosin filaments and overlapping actin filaments.
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