In an enzyme catalysed reaction, rate of reaction will keep on increasing with the increase in

Options

A. temperature.
B. enzyme concentration
C. substrate concentration
D. pH

Answer

To solve the question regarding the factors affecting the rate of an enzyme-catalyzed reaction, we can break it down into the following steps:

### Step-by-Step Solution:

1. **Understanding Enzyme-Catalyzed Reactions**:
- Enzymes are biological catalysts that increase the rate of chemical reactions by lowering the activation energy required for the reaction to occur.

2. **Identifying the Factors**:
- The question lists four factors: temperature, enzyme concentration, substrate concentration, and pH. We need to determine which of these factors will cause a continuous increase in the rate of reaction.

3. **Analyzing Enzyme Concentration**:
- As enzyme concentration increases, more active sites become available for substrate binding. This leads to a higher rate of reaction because more substrate molecules can be converted into products simultaneously.

4. **Graphical Representation**:
- If we were to plot a graph with enzyme concentration on the x-axis and the rate of reaction on the y-axis, we would see a direct linear relationship initially. As enzyme concentration increases, the rate of reaction also increases until it reaches a maximum point (saturation).

5. **Considering Other Factors**:
- While substrate concentration can also affect the rate of reaction, it does not continuously increase the rate indefinitely. Once all enzyme active sites are occupied, adding more substrate will not increase the rate further.
- Temperature and pH can affect enzyme activity, but they have optimal ranges. Beyond these ranges, the enzyme may denature or become inactive, thus not leading to a continuous increase in reaction rate.

6. **Conclusion**:
- Therefore, the factor that will keep increasing the rate of reaction in an enzyme-catalyzed reaction is **enzyme concentration**.

### Final Answer:
The rate of reaction will keep on increasing with the increase in **enzyme concentration**.

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