Man and mouse cells are treated with red and green fluorescent dyes separately and are made to fuse. The resultant cell ls when kept at `37^(@)C`, the distribution of dye on the surface of cell will be

Options

A.
B.
C.
D.

Answer

To solve the question regarding the distribution of fluorescent dyes on the surface of a fused cell from man and mouse cells, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Experiment**:
- Man and mouse cells are treated with red and green fluorescent dyes, respectively. After treatment, these cells are fused together.

2. **Temperature Consideration**:
- The resultant fused cell is kept at 37°C, which is the physiological temperature for mammalian cells. This temperature allows for normal cellular functions, including the movement of proteins within the cell membrane.

3. **Fluorescence Recovery After Photobleaching (FRAP)**:
- The technique used to analyze the distribution of the dyes is called FRAP. In this technique, the fluorescent dyes are bleached using a laser, and the recovery of fluorescence is observed over time. This helps in studying the mobility of the membrane proteins.

4. **Initial Distribution of Dyes**:
- Right after fusion, the red and green dyes would initially be segregated, with red on one side (man cells) and green on the other (mouse cells).

5. **Diffusion of Dyes**:
- After the fusion and exposure to 37°C, the proteins (and thus the dyes) will start to diffuse laterally within the membrane. This diffusion is due to the fluid mosaic model of the cell membrane, where proteins can move freely within the lipid bilayer.

6. **Expected Pattern of Distribution**:
- As the dyes diffuse, they will mix together. The expected pattern after sufficient time would be an alternating arrangement of red and green dyes due to their lateral diffusion. This would result in a pattern like red-green-red-green on the surface of the cell.

7. **Choosing the Correct Answer**:
- Based on the above understanding, the correct answer would be the one that depicts this alternating pattern of red and green fluorescence.

### Conclusion:
The distribution of the dye on the surface of the fused cell at 37°C will be in an alternating pattern of red and green (red-green-red-green). Therefore, the correct answer is option D.

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