Which of the following technique is used to measure the hormones, their precursor, and their metabolic and products quantitatively in the living body ?

Options

A. RIA
B. Chromatography
C. Oscilloscope
D. None of these

Answer

To determine which technique is used to measure hormones, their precursors, and their metabolic products quantitatively in the living body, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Hormones**:
- Hormones are biochemical substances produced in the body that regulate various physiological processes. They can be derived from amino acids or steroids.

2. **Identifying Measurement Techniques**:
- There are various techniques used to measure hormones, such as Enzyme-Linked Immunosorbent Assay (ELISA), Western Blotting, and Radioimmunoassay (RIA).

3. **Explaining Radioimmunoassay (RIA)**:
- RIA is a sensitive technique that uses radioactively labeled hormones to detect and quantify hormone levels in a sample. It involves the use of antibodies that specifically bind to the hormone of interest.

4. **Process of RIA**:
- In RIA, a sample containing the hormone is mixed with a known quantity of radioactively labeled hormone and specific antibodies. The labeled and unlabeled hormones compete for binding sites on the antibodies.
- The amount of radioactivity measured in the bound fraction is inversely proportional to the concentration of the hormone in the sample.

5. **Conclusion**:
- Based on the explanation, RIA (Radioimmunoassay) is the correct technique used to measure hormones, their precursors, and their metabolic products quantitatively in the living body.

### Final Answer:
The technique used to measure hormones, their precursors, and their metabolic products quantitatively in the living body is **Radioimmunoassay (RIA)**.

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