Which of the following dyes can be used to visualize nucleic acid after electrophoresis ?

Options

A. a.Acridine orange
B. b.Ethidium bromide
C. c.Bromophenol blue
D. d.Both (1) and (2)

Answer

To answer the question "Which of the following dyes can be used to visualize nucleic acid after electrophoresis?", we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Purpose of Dyes in Electrophoresis**:
- Dyes are used in electrophoresis to visualize nucleic acids (DNA or RNA) after they have been separated by size.

2. **Review the Given Options**:
- The options provided are:
- A) Acridine orange
- B) Ethidium bromide
- C) Bromophenol blue
- D) Both 1 and 2 (A and B)

3. **Evaluate Each Dye**:
- **Acridine Orange**: This dye can intercalate with nucleic acids and can be used for visualization, but it is less commonly used compared to others.
- **Ethidium Bromide**: This is a well-known nucleic acid stain that intercalates between the bases of DNA. It fluoresces under UV light, making it a popular choice for visualizing DNA after electrophoresis.
- **Bromophenol Blue**: This dye is primarily used as a tracking dye during electrophoresis and does not stain nucleic acids for visualization purposes.

4. **Determine the Correct Answer**:
- Since Ethidium Bromide is specifically used for visualizing nucleic acids and Acridine Orange can also be used, the best answer from the options provided is:
- **D) Both 1 and 2 (Acridine Orange and Ethidium Bromide)**.

### Final Answer:
The correct answer is **D) Both 1 and 2** (Acridine Orange and Ethidium Bromide).

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