During gel electrophoresis for separation of DNA fragment :

Options

A. Smallest fragment will move to the farthest point towards cathode
B. Smallest fragment will move to the farthest point towards anode
C. Largest fragment will move to the farthest point towards cathode
D. Largest fragment will move to the farthest point towards anode

Answer

**Step-by-Step Solution:**

1. **Understanding Gel Electrophoresis**: Gel electrophoresis is a technique used to separate DNA fragments based on their size. The gel acts as a medium through which the DNA fragments can migrate when an electric current is applied.

2. **Loading the DNA Samples**: DNA samples are loaded into wells at one end of the gel. The gel is typically made of agarose or polyacrylamide, which creates a matrix for the DNA to move through.

3. **Applying Electric Current**: When an electric field is applied, the negatively charged DNA fragments will migrate towards the positive electrode, known as the anode.

4. **Movement of DNA Fragments**: The movement of DNA fragments through the gel depends on their size. Smaller fragments can navigate through the pores of the gel more easily and therefore move faster than larger fragments.

5. **Determining the Farthest Point**: As a result, the smallest DNA fragments will travel the farthest distance towards the anode, while the larger fragments will not move as far.

6. **Conclusion**: Therefore, the correct answer to the question is that the smallest fragment will move to the farthest point towards the anode.

**Final Answer**: The smallest fragment will move to the farthest point towards the anode.

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