For transformation with recombinan DNA, the bacterial cells must first be made compentent which means

Options

A. Should increase their metabolic reactions
B. Should decrease their metabolic reactions
C. Increase efficiency with which DNA enters the bacterium
D. Ability to divide fast

Answer

To answer the question regarding the transformation of bacterial cells with recombinant DNA, we need to understand what it means for bacterial cells to be made competent. Here’s a step-by-step breakdown:

### Step 1: Understanding Competence
- Competence refers to the ability of bacterial cells to take up foreign DNA from their environment. This is a critical step in the process of transformation, where DNA is introduced into a bacterial cell.

### Step 2: Importance of Extracellular DNA
- Bacterial cells can naturally take up extracellular DNA, which is DNA that originates outside the cell. This DNA can be from other bacteria or from recombinant DNA that researchers want to introduce into the cells.

### Step 3: Increasing Efficiency
- For a bacterial cell to be competent, it must have an increased efficiency in taking up this extracellular DNA. This means that the cell's membrane must be altered or treated in such a way that it allows DNA to enter more readily.

### Step 4: Methods of Making Bacteria Competent
- Bacteria can be made competent through various methods, such as:
- Chemical treatment (e.g., using calcium chloride)
- Physical methods (e.g., electroporation)
- Natural methods (some bacteria are naturally competent)

### Step 5: Conclusion
- Therefore, when the question states that bacterial cells must be made competent for transformation with recombinant DNA, it means that the cells must be treated or modified to enhance their ability to uptake DNA efficiently.

### Final Answer
- For transformation with recombinant DNA, the bacterial cells must first be made competent, which means they should have increased efficiency in taking up the DNA.

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