Isolation of the genetic material in pure form, free from other macromolecules can be achieved by treating the bacterial cells/plant or animal tissues with the following enzymes, except

Options

A. Lysozyme
B. Cellulase
C. Chitinase
D. Ligase

Answer

To solve the question regarding the isolation of genetic material in pure form from bacterial cells or plant/animal tissues, we need to analyze the role of each enzyme mentioned in the options.

### Step-by-Step Solution:

1. **Understanding the Question**: The question asks which enzyme does NOT help in isolating genetic material by breaking down other macromolecules in bacterial cells or plant/animal tissues.

2. **Analyzing the Options**:
- **Option 1: Lysozyme**
- Lysozyme is an enzyme that breaks down peptidoglycan in bacterial cell walls. It is found in various body fluids, including tears. This enzyme helps in the digestion of bacterial cell walls, facilitating the release of genetic material.
- **Option 2: Cellulase**
- Cellulase is an enzyme that breaks down cellulose, which is a major component of plant cell walls. By digesting cellulose, cellulase helps release genetic material from plant tissues.
- **Option 3: Chitinase**
- Chitinase is an enzyme that digests chitin, a component of fungal cell walls. It is useful for isolating genetic material from fungal cells.
- **Option 4: Ligase**
- Ligase is an enzyme that joins two DNA fragments together. It does not have a role in breaking down cell walls or digesting macromolecules. Its primary function is in DNA replication and repair, not in the isolation of genetic material.

3. **Conclusion**: Since lysozyme, cellulase, and chitinase are all involved in breaking down cell walls and facilitating the release of genetic material, while ligase does not perform this function, the correct answer to the question is **Option 4: Ligase**.

### Final Answer:
The enzyme that does NOT help in the isolation of genetic material in pure form is **Ligase** (Option 4).

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