Denaturation can be achieved at which temperature during PCR?

Options

A. `72^(@)C`
B. `95^(@)C`
C. `40^(@)C`
D. `25^(@)C`

Answer

### Step-by-Step Solution:

1. **Understanding PCR**: PCR stands for Polymerase Chain Reaction, a technique used to amplify DNA. It involves a series of temperature changes that allow for the replication of DNA.

2. **Identifying the Processes in PCR**: PCR consists of three main processes:
- **Denaturation**: The double-stranded DNA is separated into two single strands.
- **Annealing**: Primers bind to the single-stranded DNA.
- **Extension**: New DNA strands are synthesized.

3. **Focusing on Denaturation**: The question specifically asks about the temperature at which denaturation occurs.

4. **Temperature Range for Denaturation**: Denaturation typically occurs at a temperature range of **94 to 98 degrees Celsius**.

5. **Choosing the Correct Option**: Among the given options:
- 72 degrees Celsius
- 95 degrees Celsius
- 40 degrees Celsius
- 25 degrees Celsius

The option that falls within the denaturation temperature range is **95 degrees Celsius**.

6. **Conclusion**: Therefore, the correct answer to the question is **95 degrees Celsius**.