Choose the incorrect statement with respect to Agrobacterium tumefaciens.

Options

A. a.It is a Gram-negative soil bacterium.
B. b.It produces crown gall disease in dicot plants.
C. c.The foreign DNA is inserted at the ori site of Ti plasmid.
D. d.Ti plasmid becomes incorporated into the plant chromosomal DNA.

Answer

To solve the question regarding Agrobacterium tumefaciens and identify the incorrect statement, follow these steps:

### Step-by-Step Solution:

1. **Understand Agrobacterium tumefaciens**:
- It is a rod-shaped, gram-negative bacterium found in soil.
- It is known for causing crown gall disease in dicot plants by inducing tumors.

2. **Review the Mechanism of Action**:
- Agrobacterium tumefaciens transfers a segment of its DNA, known as T-DNA, into the plant cells.
- This T-DNA integrates into the host plant's genome, leading to tumor formation.

3. **Analyze the Options**:
- **Option A**: "It is a gram negative soil bacterium."
- This statement is **true**.
- **Option B**: "It produces crown gall disease in dicot plants."
- This statement is **true**.
- **Option C**: "The foreign DNA is inserted at the ori site of TI plasmid."
- This statement is **false**. The T-DNA does not insert at the ori site; it integrates into the host genome.
- **Option D**: "The diplasmid becomes incorporated into the plant chromosomal DNA."
- This statement is **true**.

4. **Identify the Incorrect Statement**:
- Based on the analysis, **Option C** is the incorrect statement regarding Agrobacterium tumefaciens.

### Final Answer:
The incorrect statement with respect to Agrobacterium tumefaciens is **Option C**: "The foreign DNA is inserted at the ori site of TI plasmid."

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