If the gene of interest is cloned at ECo...

If the gene of interest is cloned at ECoRI in pBR322, the recombinant E-coli after transformation are

Ampicillin

Tetracycline

Both (1) and (2)

Kanamycin

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The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

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