I. Transform bacteria with recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacteria cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V. Use ligate to seal plasmid DNA to non-plasmid DNA.
From the given list, which of the following is the most logical sequence of steps for splicing foregain DNA into a plasmid and inserting the plasmid into a bacterium ?

Options

A. IV,V,I,II,III
B. III,II,IV,V,I
C. III,IV,V,I,II
D. II,III,V,IV,I

Answer

To determine the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium, we can analyze the provided steps and arrange them in a coherent order. Here’s the step-by-step solution:

### Step-by-Step Solution:

1. **Extract plasmid DNA from bacterial cells.**
This is the first step because we need to obtain the plasmid that will be modified.

2. **Cut the plasmid DNA using restriction enzymes.**
After extracting the plasmid, we use restriction enzymes to cut the plasmid DNA at specific sites. This prepares the plasmid for the insertion of foreign DNA.

3. **Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.**
Once the plasmid is cut, we can introduce the foreign DNA fragments. Hydrogen bonds will form between the complementary ends of the plasmid and the foreign DNA.

4. **Use ligase to seal plasmid DNA to non-plasmid DNA.**
After the foreign DNA is attached, ligase is used to seal the nicks in the sugar-phosphate backbone, creating a stable recombinant DNA molecule.

5. **Transform bacteria with recombinant DNA molecule.**
Finally, the recombinant plasmid is introduced into bacterial cells through a process called transformation, allowing the bacteria to express the new genetic material.

### Final Sequence:
- III. Extract plasmid DNA from bacteria cells.
- II. Cut the plasmid DNA using restriction enzymes.
- IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
- V. Use ligase to seal plasmid DNA to non-plasmid DNA.
- I. Transform bacteria with recombinant DNA molecule.