Which of the following enzymes cut the DNA molecule at specific nucleotide sequence ?

Options

A. Restriction endonuclease
B. DNA ligase
C. RNA polymerase
D. Exonuclease

Answer

To solve the question "Which of the following enzymes cut the DNA molecule at specific nucleotide sequence?", we will analyze each of the given options step by step.

### Step-by-Step Solution:

1. **Understanding the Question**: The question asks for an enzyme that specifically cuts DNA at certain nucleotide sequences. This implies we are looking for an enzyme that has specificity in its action.

2. **Analyzing the Options**:
- **Option A: Restriction Endonuclease**: This enzyme cuts DNA at specific sequences. The term "restriction" indicates that it acts at particular sites within the DNA molecule. Endonucleases cut the DNA strand at internal locations, which aligns with the requirement of the question.

- **Option B: DNA Ligase**: This enzyme is responsible for joining DNA fragments together. It does not cut DNA; instead, it facilitates the ligation of DNA strands. Therefore, it does not fit the criteria of the question.

- **Option C: RNA Polymerase**: This enzyme synthesizes RNA from a DNA template during transcription. It does not cut DNA; it is involved in the process of copying DNA into RNA. Hence, it is not the correct answer.

- **Option D: Exonuclease**: Exonucleases are enzymes that remove nucleotides from the ends of a DNA molecule. They cut DNA from the ends rather than at specific internal sequences, making them the opposite of what the question is asking.

3. **Conclusion**: After analyzing all the options, the only enzyme that cuts DNA at specific nucleotide sequences is **Restriction Endonuclease**.

### Final Answer:
The correct answer is **Restriction Endonuclease**.

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