Which of the followoing restriction endonuclease enzyme produce blunt end in DNA :

Options

A. `{:(,,darr,,,,,),("Bam","HI",G,G,A,T,C,C),(,,C,C,T,A,G,G),(,,,,,,uarr,):}`
B. `{:(,,,darr,,,,),("Eco","RI",G,A,A,T,T,C),(,,C,T,T,A,A,G),(,,,,,,uarr,):}`
C. `{:(,,,,darr,),("Hae","III",G,G,C,C),(,,C,C,G,G),(,,,,uarr,):}`
D. All the above

Answer

To determine which of the following restriction endonuclease enzymes produces blunt ends in DNA, we need to analyze the cutting patterns of the given enzymes: BamH1, EcoR1, and HaeIII.

### Step-by-Step Solution:

1. **Understanding Restriction Endonucleases**:
- Restriction endonucleases are enzymes that cut DNA at specific recognition sites. They can produce two types of ends: sticky ends (staggered cuts) and blunt ends (non-staggered cuts).

2. **Analyzing BamH1**:
- BamH1 recognizes the sequence G^GATCC (the caret symbol indicates the cut site).
- It cuts between the two G bases, resulting in sticky ends because the cut is staggered (one strand is longer than the other).

3. **Analyzing EcoR1**:
- EcoR1 recognizes the sequence G^AATTC.
- It cuts between G and A, also producing sticky ends due to the staggered cut.

4. **Analyzing HaeIII**:
- HaeIII recognizes the sequence G^GCC.
- It cuts between the two G bases, producing blunt ends because the cut is straight across both strands without leaving any overhang.

5. **Conclusion**:
- Among the enzymes listed, only HaeIII produces blunt ends. Therefore, the answer to the question is HaeIII.

### Final Answer:
The restriction endonuclease enzyme that produces blunt ends in DNA is **HaeIII**.