The technique of transferring DNA fargment separated on agarose gel to a synthetic membrane such as nitrocellulose is known as

Options

A. Northern blotting
B. Southern blotting
C. Western blotting
D. Dot blotting

Answer

To answer the question regarding the technique of transferring DNA fragments separated on agarose gel to a synthetic membrane such as nitrocellulose, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Blotting Techniques**: Blotting is a technique used to transfer DNA, RNA, or protein molecules from a gel to a membrane. This process allows for the separation and identification of these biomolecules.

2. **Identifying the Types of Blotting**:
- **Northern Blotting**: This technique is specifically used for the transfer of RNA fragments. Since the question asks about DNA, this option is not correct.
- **Southern Blotting**: This technique is used for transferring specific DNA fragments from an agarose gel to a synthetic membrane, such as nitrocellulose. This matches the requirement of the question.
- **Western Blotting**: This method is used for detecting proteins, not DNA. Therefore, it is not the correct answer.
- **Dot Blotting**: This is a simpler technique used for detecting biomolecules but does not specifically refer to the transfer of DNA from agarose gel to a membrane.

3. **Conclusion**: Based on the definitions and uses of the different blotting techniques, the correct answer to the question is **Southern Blotting**. This technique specifically refers to the transfer of DNA fragments from an agarose gel to a nitrocellulose membrane.

### Final Answer:
The technique of transferring DNA fragments separated on agarose gel to a synthetic membrane such as nitrocellulose is known as **Southern Blotting**.