A: Taq Polymerase is involved in PCR technique.
R: This enzyme remain active during the high temperature including denaturation of double stranded DNA.

Options

A. If both Assertion and Reason are true and the reason is the correct explanation of the assertion, then mark (1).
B. If both Assertion and Reason are true but the reason is not the correct explanation of the assertion then mark (2).
C. If Assertion is true but Reason are false, then mark(3).
D. If both Assertion and Reason are false, then mark(4).

Answer

### Step-by-Step Text Solution:

1. **Understanding the Assertion**: The assertion states that "Taq Polymerase is involved in PCR technique." This is true as Taq Polymerase is a crucial enzyme used in the Polymerase Chain Reaction (PCR) process.

2. **Understanding the Reason**: The reason provided is "This enzyme remains active during the high temperature including denaturation of double-stranded DNA." This is also true. Taq Polymerase is known for its heat stability, allowing it to function effectively at high temperatures.

3. **Explaining PCR**: PCR is a technique used to amplify DNA, and it involves repeated cycles of denaturation, annealing, and extension. Taq Polymerase plays a vital role during the extension phase, where it synthesizes new DNA strands.

4. **Heat Stability of Taq Polymerase**: Taq Polymerase is isolated from the thermophilic bacterium Thermus aquaticus, which thrives in high-temperature environments. This characteristic allows the enzyme to remain active even at temperatures that would denature other enzymes.

5. **Optimal Temperature for Activity**: The optimal temperature for Taq Polymerase activity is between 72°C to 75°C. At this temperature, the enzyme exhibits maximum efficiency in synthesizing DNA.

6. **Conclusion**: Since both the assertion and the reason are true, and the reason accurately explains why the assertion is true, we conclude that both statements are correct and that the reason is equal to the assertion.

### Final Answer:
Both the assertion and reason are true, and the reason is equal to the assertion.

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