Thermal cycle takes place in which technique

Options

A. Gel electrophoresis
B. PCR technique
C. Centrifugation
D. Southern blotting

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks which technique involves a thermal cycle. A thermal cycle typically refers to a process that includes repeated heating and cooling steps.

2. **Analyzing the Options**:
- **Option 1: Gel Electrophoresis**: This technique is used to separate DNA fragments based on size. It involves applying an electric current to a gel, but does not involve any heating or cooling cycles. Therefore, this option is incorrect.

- **Option 2: PCR Technique**: PCR stands for Polymerase Chain Reaction. This technique is specifically designed to amplify DNA and involves repeated cycles of heating (denaturation) and cooling (annealing and extension). This is the definition of a thermal cycle, making this option correct.

- **Option 3: Centrifugation Technique**: This method is used to separate particles from a solution based on size, shape, and density. It does not involve any thermal cycling, so this option is also incorrect.

- **Option 4: Southern Blotting**: This is a method used for detecting specific DNA sequences. It involves gel electrophoresis and does not include any thermal cycling. Thus, this option is incorrect as well.

3. **Conclusion**: The only technique that involves a thermal cycle is the PCR technique. Therefore, the correct answer is **PCR Technique**.

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